40818 DATASHEET PDF

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Prepare solutions with reverse osmosis deionized RODI or equivalently purified water. If using whole blood, lyse red blood cells and wash by centrifugation prior to fixation.

Phospho-STING (Ser366) (D8K6H) Rabbit mAb #40818

Count cells using a hemocytometer or alternative method. Scrape cells off the plate and transfer to microcentrifuge tubes. Pre-wash magnetic beads just prior to use: Treat cells by adding fresh media containing regulator for desired time. This protocol is intended for immunoprecipitation of native proteins for analysis by western immunoblot or kinase activity utilizing Protein A magnetic separation.

Remove PBS and add 0. Appropriate isotype controls are highly recommended in order to show specific binding in your primary antibody immunoprecipitation. Proceed to immunoprecipitation section.

Carefully remove the buffer once the solution is clear.

Would you like to visit your country specific website? Recommended Fluorochrome-conjugated Anti-Rabbit secondary antibodies: For best results, allow mountant 4018 cure overnight at room temperature. Dilute to 1X with dH 2 O. Isotype controls should be concentration matched and run alongside the primary antibody samples. Briefly vortex the stock tube to resuspend the magnetic beads.

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Adjust pH to 8. To prepare 10 ml, add 0. Immunoprecipitation Cell Lysate Pre-Clearing Optional A cell lysate pre-clearing step is highly recommended to reduce non-specific protein binding to the Protein A Magnetic beads. Pellet beads using magnetic separation rack. Find answers on our FAQs page. A cell lysate pre-clearing step is highly recommended to reduce non-specific protein binding to the Protein Datashheet Magnetic beads.

Aspirate blocking solution, apply diluted primary antibody. The optimal lysate concentration will depend on the expression level of the protein of interest.

Wash cells by centrifugation in excess 1X PBS to remove methanol. Changing to another country might result in loss of shopping cart.

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Vortex, then microcentrifuge for 30 sec. Pre-wash magnetic beads see Cell Lysate Pre-Clearing section, steps 1 and 2. More about how we get our images. Incubate membrane in 25 ml of blocking buffer for 1 hr at room temperature. Incubate substrate with membrane for 1 minute, remove excess solution membrane remains wetwrap in 48018 and expose to X-ray film. Monoclonal antibody is produced datsaheet immunizing animals with a synthetic peptide corresponding to residues surrounding Ser of human STING protein.

Biotinylated Protein Ladder Detection Pack: Sonicate on ice three times for 5 sec each. This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody.

Solutions and Reagents From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: Would you like to visit your country specific website? Analyze cells in DNA staining solution on flow cytometer.

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Sample Analysis Proceed to one of the following specific set of steps. USP8 Antibody – Application Dilutions Immunofluorescence Immunocytochemistry 1: Please refer to primary antibody datasheet or product webpage for recommended antibody dilution.

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Solutions and Reagents Achieve higher quality immunofluorescent images using the efficient and cost-effective, pre-made reagents in our Immunofluorescence Application Solutions Kit NOTE: Aspirate media from cultures; wash cells with 1X PBS; aspirate. Mix well then add 0. Cells should be grown, treated, fixed and stained directly in multi-well plates, chamber slides or on coverslips. Dagasheet about how we get our images.

It should be noted that for the best possible results a fresh blot is always recommended.

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Incubate for 1 hr at room temperature. The supernatant is the sample. Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available. Prepare solutions with reverse osmosis deionized RODI or equivalent grade water.

Collect cells by centrifugation and aspirate supernatant.